Novel Molecules for Intra-Oral Delivery of Antimicrobials to Prevent and Treat Oral Infectious Diseases

New molecules were designed for efficient intra-oral delivery of antimicrobials to prevent and treat oral infection. The salivary statherin fragment, which has high affinity for the tooth enamel, was used as a carrier peptide. This was linked through the side chain of the N-terminal residue to the C-terminus of a defensin-like 12-residue peptide to generate two bifunctional hybrid molecules, one with an ester linkage and the other with an anhydride bond between the carrier and the antimicrobial components. They were examined for their affinity to a HAP (hydroxyapatite) surface. The extent of the antimicrobial release in human whole saliva was determined using 13C-NMR spectroscopy. The candidacidal activity of the molecules was determined as a function of the antimicrobial release from the carrier peptide in human saliva. The hybrid-adsorbed HAP surface was examined against Candida albicans and Aggregatibacter actinomycetemcomitans using the fluorescence technique. The bifunctional molecules were tested on human erythrocytes, GECs (gingival epithelial cells) and GFCs (gingival fibroblast cells) for cytotoxicity. They were found to possess high affinity for the HAP mineral. In human whole saliva, a sustained antimicrobial release over a period of more than 40–60 h, and candidacidal activity consistent with the extent of hybrid dissociation were observed. Moreover, the bifunctional peptide-bound HAP surface was found to exhibit antimicrobial activity when suspended in clarified human saliva. The hybrid peptides did not show any toxic influence on human erythrocytes, GECs and GFCs. These novel hybrids could be safely used to deliver therapeutic agents intra-orally for the treatment and prevention of oral infectious diseases

FireFly Mosaic: A Vision-Enabled Wireless Sensor Networking System

Abstract — With the advent of CMOS cameras, it is now possible to make compact, cheap and low-power image sensors capable of on-board image processing. These embedded vision sensors provide a rich new sensing modality enabling new classes of wireless sensor networking applications. In order to build these applications, system designers need to overcome challanges associated with limited bandwith, limited power, group coordination and fusing of multiple camera views with various other sensory inputs. Real-time properties must be upheld if multiple vision sensors are to process data, com-municate with each other and make a group decision before the measured environmental feature changes. In this paper, we present FireFly Mosaic, a wireless sensor network image processing framework with operating system, networking and image processing primitives that assist in the development of distributed vision-sensing tasks. Each FireFly Mosaic wireless camera consists of a FireFly [1] node coupled with a CMUcam3 [2] embedded vision processor. The FireFly nodes run the Nano-RK [3] real-time operating system and communicate using the RT-Link [4] collision-free TDMA link protocol. Using FireFly Mosaic, we demonstrate an assisted living application capable of fusing multiple cameras with overlapping views to discover and monitor daily activities in a home. Using this application, we show how an integrated platform with support for time synchronization, a collision-free TDMA link layer, an underlying RTOS and an interface to an embedded vision sensor provides a stable framework for distributed real-time vision processing. To the best of our knowledge, this is the first wireless sensor networking system to integrate multiple coordinating cameras performing local processing. I

RT-Link: A Time-Synchronized Link Protocol for Energy-Constrained Multi-hop Wireless Networks

We propose RT-Link, a time-synchronized link protocol for real-time wireless communication in industrial control, surveillance and inventory tracking. RT-Link provides predictable lifetime for battery-operated embedded nodes, bounded end-to-end delay across multiple hops, and collision-free operation. We investigate the use of hardwarebased time-synchronization for infrastructure nodes by using an AM carrier-current radio for indoors and atomic clock receivers for outdoors. Mobile nodes are synchronized via in-band software synchronization within the same framework. We identify three key observations in the design and deployment of RT-Link: (a) Hardware-based global-time synchronization is a robust and scalable option to in-band softwarebased techniques. (b) Achieving global time-synchronization is both economical and convenient for indoor and outdoor deployments. (c) RT-Link achieves a practical lifetime of over 2 years. Through analysis and simulation, we show that RTLink outperforms energy-efficient link protocols such as BMAC in terms of node lifetime and end-to-end latency. The protocol supports flexible services such as on-demand endto- end rate control and logical topology control. We implemented RT-Link on the CMU FireFly sensor platform and have integrated it within the nano-RK real-time sensor OS. A 42-node network with sub-20us synchronization accuracy has been deployed for 3 weeks in the NIOSH Mining Research Laboratory and within two 5-story campus buildings

Recovery Time Considerations in Real-Time Systems Employing Software Fault Tolerance

Safety-critical real-time systems like modern automobiles with advanced driving-assist features must employ redundancy for crucial software tasks to tolerate permanent crash faults. This redundancy can be achieved by using techniques like active replication or the primary-backup approach. In such systems, the recovery time which is the amount of time it takes for a redundant task to take over execution on the failure of a primary task becomes a very important design parameter. The recovery time for a given task depends on various factors like task allocation, primary and redundant task priorities, system load and the scheduling policy. Each task can also have a different recovery time requirement (RTR). For example, in automobiles with automated driving features, safety-critical tasks like perception and steering control have strict RTRs, whereas such requirements are more relaxed in the case of tasks like heating control and mission planning. In this paper, we analyze the recovery time for software tasks in a real-time system employing Rate-Monotonic Scheduling (RMS). We derive bounds on the recovery times for different redundant task options and propose techniques to determine the redundant-task type for a task to satisfy its RTR. We also address the fault-tolerant task allocation problem, with the additional constraint of satisfying the RTR of each task in the system. Given that the problem of assigning tasks to processors is a well-known NP-hard bin-packing problem we propose computationally-efficient heuristics to find a feasible allocation of tasks and their redundant copies. We also apply the simulated annealing method to the fault-tolerant task allocation problem with RTR constraints and compare against our heuristics

Voice Over Sensor Networks

Wireless sensor networks have traditionally focused on low duty-cycle applications where sensor data are reported periodically in the order of seconds or even longer. This is due to typically slow changes in physical variables, the need to keep node costs low and the goal of extending battery lifetime. However, there is a growing need to support real-time streaming of audio and/or low-rate video even in wireless sensor networks for use in emergency situations and shortterm intruder detection. In this paper, we describe a real-time voice stream-capability in wireless sensor networks and summarize our deployment experiences of voice streaming across a large sensor network of FireFly nodes in an operational coal mine. FireFly is composed of several integrated layers including specialized low-cost hardware, a sensor network operating system, a real-time link layer and network scheduling. We are able to provide efficient support for applications with timing constraints by tightly coupling the network and task scheduling with hardware-based global time synchronization. We use this platform to support 2-way audio streaming concurrently with sensing tasks. For interactive voice, we investigate TDMA-based slot scheduling with balanced bi-directional latency while meeting audio timeliness requirements. Finally, we describe our experimental deployment of 42 nodes in a coal mine, and present measurements of the end-to-end throughput, jitter, packet loss and voice quality

Opportunities and obligations for physical computing systems

The recent confluence of embedded and real-time systems with wireless, sensor, and networking technologies is creating a nascent infrastructure for a technical, economic, and social revolution. Based on the seamless integration of computing with the physical world via sensors and actuators, this revolution will accrue many benefits. Potentially, its impact could be similar to that of the current Internet. We believe developers must focus on the physical, real-time, and embedded aspects of pervasive computing. We refer to this domain as physical computing systems. For pervasive computing to achieve its promise, developers must create not only high-level system software and application solutions, but also low-level embedded systems solutions. To better understand physical computing\u27s advantages, we consider three application areas: assisted living, emergency response systems for natural or man-made disasters, and protecting critical infrastructures at the national level

Study of tensile deformation behaviour of M250 grade maraging steel using acoustic emission

Tensile testing of solution annealed and thermally aged (755 K for various durations in the range of 0.25-100 h) specimens of M250 grade maraging steel has been carried out along with acoustic emission (AE) monitoring. Results have shown that strength increases and ductility decreases upon ageing up to 10 h and this has been attributed primarily to the precipitation of Ni3Ti. Continued increase in strength up to 40 h of ageing has been attributed primarily to the precipitation of Fe2Mo in addition to Ni3Ti. Increase in ductility for 10-40 h of ageing has been attributed to dissolution of needle like Ni3Ti precipitates and formation of fine spherical Fe2Mo. Ageing beyond 40 h decreases strength and increases ductility due to the reversion of martensite to austenite and coarsening of the precipitates. The AE generated during tensile deformation depends on the ageing time. Increased occurrence of shearing of the precipitates by dislocations and increased brittleness of the matrix up to 10 h ageing increases the AE. The decrease in the AE beyond 10 h of ageing is due to the occurrence of deformation by Orowan looping, dissolution of Ni3Ti precipitates and austenite reversion. The scanning electron microscopy (SEM) of the fracture surfaces has shown ductile fracture characterized by dimples and changes in the size and shape of the dimples with ageing time

Bounded-Latency Alerts in Vehicular Networks

Vehicle-to-vehicle communication protocols may be broadly classified into in three categories; bounded-delay safety alerts, persistent traffic warnings and streaming media for telematics applications. We focus on the first category of time-critical messaging as is it of greatest value to the driver and passengers. Safety alerts are transmitted from a vehicle during events such as loss of traction, sudden braking and airbag deployment. The objective for a safety protocol is to relay messages across multiple vehicles within a 1.5-2km distance to alert approaching vehicles within a bounded end-to-end delay (e.g. 1.5 sec). Due to high mobility and ephemeral connectivity we must employ broadcast protocols, as well as mitigation strategies to curtail inherent issues associated with broadcast protocols, such as broadcast storm problem. In this paper, we present a Location Division Multiple Access (LDMA) scheme to suppress the broadcast storm problem and ensure bounded end-to-end delay across multiple hops. This scheme requires participating vehicles to time synchronize with the GPS time and receive the regional map definitions consisting of spatial cell resolutions and temporal slot schedules via an out-of-band FM/RDBS control channel. We use the GrooveNet vehicular network virtualization platform with realistic mobility, car-following and congestion models to evaluate the performance of LDMA in simulation and on the road